对四氯化碳所致大鼠肝脏损伤机制的研究

A Study on Mechanism of Liver Lesion Induced by Carbon Tetrachloride in Rats

采用四氯化碳(CCl _4)中毒性大鼠肝损害模型,用薄层层析和定磷法检测四组大鼠肝脏膜磷脂成份磷脂酰胆碱(Phosphatidylcholne PC),鞘磷脂(Sphingomyelin,SM)在总磷脂中的相对百分含量,并测定肝脏组织内的γ-谷氨酰转肽酶(γGT)的活性。结果表明:在CCl_4染毒后12和24小时,PC和SM明显降低,48小时又有所上升。但PC尽管回升,与对照组比较仍低(P<0.05),而SM和对照组之间无显著性差别。γ-GT的活性在12、24、48h分别为对照组的29％、44.7％、147％,与对照组之间的差别有显著性。这提示CCl_4染毒后,PC和SM的变化有类似于癌变过程中PC扣SM变化的趋势,可能具有相似的调控作用。

Twenty male rats were classified into four groups among which a group as control were decapitated and the other three were decapitated for livers in 12, 24 and 48 hours of rat's intoxication by carbon tetrachloride (CC14) (2. 5ml/kg body weight, I. G. ), respectively. The livers were tested for the relative weight of phosphatidylcholine (PC) and sphingmyelin (SM) in the total amount of membrane phospholipid using thin-layer chromatography (TCL) and determination of phospholipid phosphorus content. Meanwhile the activity of Gamma-glutamine transferase (GGT) was measured in the liver tissues. The results showed that PC and SM decreased significantly at 12 and 24 hours after the administration of CC14, but at 48 hours resynthesized approximately to that of control group except that PC at 48 hours, comparable with that of control, was still lower (P<0. 05). The activity of GGT at 12, 24 and 48 hours was 29%, 44.7% and 147% of that in control respectively (P< 0. 05). This suggested that the tendency of PC and SM alternation in the liver of rat intoxicated by CC14 was similar to that in tumor tissues, and that indicated that both the injured liver by CCl4 and hepatoma shared the same mechanism in regulating the metabolism of PC and SM. However, it needs further studying.