系统性红斑狼疮26例IL-10及IL-12的异常表达及其相互作用

Effect of IL-10 on IL-12 production involved in the pathogenesis of SLE

目的:了解系统性红斑狼疮(SLE)患者白细胞介素(IL)-10、IL-12异常表达的相互关系,探讨IL-10对IL-12分泌的影响。方法:取26例SLE患者及正常对照18例志愿者的外周血(PBMCs),用ELISA法测定其血清、单个核细胞(PBMCs)培养上清、植物血凝素(PHA)刺激培养上清中IL-10、IL-12水平;在SLE患者及正常对照PBMCs中分别加入正常人血清、SLE血清、SLE血清+IL-10抗体,PHA刺激培养24h,用ELISA法测培养上清中IL-12的水平。结果:SLE患者血清、PBMCs培养上清中自发产生和PHA刺激诱生的IL-10及IL-12P40水平均异常增高。然而仅血清IL-10水平与SLEDAI呈正相关,但血清IL-12P40水平、PBMCs培养上清自发产生的及PHA刺激诱生的IL-12P40水平均与SLEDAI呈正相关。尽管SLE患者血清IL-10水平与IL-12P40血清及PBMCs培养上清水平呈正相关,但体外实验时,在PBMCs培养中,加入SLE血清,可显著抑制IL-12的产生,采用IL-10抗体中和掉SLE血清中的IL-10后,正常人或SLE患者的PBMCs培养上清中IL-12可明显增多。结论:SLE患者IL-10及IL-12表达水平异常增高,且血清水平与SLE活动性正相关;体外细胞培养实验中SLE血清中IL-10可明显抑制IL-12的分泌,但在细胞因子网络环境中两者之间相互作用更为复杂。

Objective： To investigate the association between the levels of IL-10 and IL-12 in the patients with systemic lupus erythematosus（SLE）, and to investigate the effect of IL-10 on the production of IL-12. Methods： ELISA was used to detect the level of IL-10 and IL-12 in serum and cultured PBMC supernatants. SLE serum was used to culture the PBMCs to show how the cytokine IL-10 in SLE serum influenced the production of IL-12. Results： The experiments showed that there were elevated IL-10 and IL-12 levels in the serum and cultured PBMCs supernatants of SLE patients. Furthermore, increased serum IL-10 had positive association with SLEDAI, and the correlation between IL-12 level in serum, cultured supernatants and disease activity were also found. When cultured with serum from SLE patients with high level of IL-10, PBMCs produced less IL-12 than control. If IL-10 neutralizing antibody was added into the culture system, decreased IL-12 could be restored.Conclusion： Increased IL-10 and IL-12 in serum of patients with SLE is positively correlated with SLEDAI. IL-10 could inhibit the production of IL-12 by PBMCs.