HCMV 60-mer寡核苷酸诊断芯片的探针与微阵列设计

60-mer oligonucleotide probes and gene arrays for human cytomegalovirus defection

目的设计用于人巨细胞病毒(HCM V)诊断的60-m er长链寡核苷酸探针及微阵列。方法利用生物学软件A rray D es igner2.0,针对HCM V特异且保守的序列设计60-m er探针,利用BLA ST功能将设计探针在G enB ank数据库进行序列比对分析,筛选得到HCM V特异性O ligo探针,根据各探针的属性特点设计芯片阵列。结果设计得到24条解链温度(Tm值)相近、长度均一的60-m er O ligo探针及其芯片阵列,拟打印成DNA芯片用于HCM V检测。结论利用病原体的生物信息学资料及A rray D es igner2.0生物软件,能有效的进行病毒检测芯片的设计。

Objectives ： To design 60-met oligonucleotide probes and gene arrays for human cytomegalovirus defection. Methods ： Array Designer 2. 0 software was applied to design 60-met oligonucleotide probes with high specificity, identical length and similar melting temperature （Tm） relevant to special gene sequence of human cytomegalovirus. Results ： Altogether 24 60-mer oligonucleotide probes were designed, selected and deposited on oligonucleotide microarrays for detection of human cytomegalovirus. Conclusions： With the biological information of pathogen and biological software Array Designer 2. 0, probes can be designed efficiently for diagnostic mieroarray of human cytomegalovirus.