疏肝健脾治疗与肝郁脾虚因素刺激对大肠癌大鼠免疫功能变化的探讨

Immunological Study on Rats with Iarge Intestine Cancer of Shuganjianpi Treatment Liver Depression and spllen deficiency

目的探讨肝郁脾虚因素刺激对大鼠大肠癌的免疫学指标。方法SD大鼠经肝郁脾虚因素刺激和DMH20mg/kg颈部皮下注射,每周1次,连续15周后停用二甲肼,建立大鼠大肠癌模型(单纯组)和肝郁脾虚型大肠癌(病症综合组),观察各组大鼠主要症状、体征、结肠粘膜损伤指数(CMD1)及大鼠细胞免疫、体液免疫、非特异性免疫功能的变化。结果SD大鼠经肝郁脾虚因素刺激和DMH作用下,单纯组、病症综合组大鼠饮食量、体重明显下降,出现粘液样血便,脾淋巴细胞增率、迟发型超敏反应(DTH)及胸腺指数明显升高(P<0.05),血清溶血素水平、脾溶血容斑细胞、脾脏指数及腹腔巨噬细胞吞噬功能有所降低。复合组以上各项指标比单纯组改变明显。结论大鼠大肠癌模型表现为细胞免疫功能亢进及体液免疫和非特异性免疫功能低下,提示其发病与机体免疫功能紊乱、神经-内分泌-免疫网络失调关系密切。

Objective To study the immunological pathogenesis Rats with large intestine cancer of liver Depression and spleen deficiency. Methods SD rats in the experiment were stimulated with the factors of liver Depression and spleen deficiency（Ldsd） and at cervix injected with DMB 20mg/kg, once a week and stop the DMH after 15 weeks; Rats model of large intestine cancer（simple） and syndrome of LDSD （complex）. To observe the main symptom, physicalsign and detect the colon mucosa damage index, the function of cell mediated immunity, antibody mediated immunity and nonspecific immunity. Results the result showed that the aooetite and the body weight of two rats models decreased obviously with serious loose stool, the transformation rate of lymphocytes , the plaque froming cell, splee index and the phagocytosis ability of maerophage were remarkably deceased. The changes of the index of rats models of large intestine cancer with the liver Depression and spleen deficiency were more obviously that those only acid induced cacer models. Conclusion Rats models showed that cell mediated immunity is hyperfunction, the antibody-mediated immunity and non-specific immunity were decreased , It suggests that pathogenesis of the large intestine cancer is correlated closely with confusion of immunologicl functoion and neurological-endocrinological-immunological system.