一种纯系小鼠动脉粥样硬化病理模型的建立

The Establishment of an Atherosclerotic Murine Model and Pathological Observation of Plaques by Laser Scanning Confocal Microscopy

实验选用Ｃ５７ＢＬ／６Ｊ纯系小鼠，饲喂含２％胆固醇饮食１６周后，血清脂质（包括总胆固醇、游离胆固醇和甘油三酯）水平升高，血清总胆固醇为３．６９±０．７８ｇ／Ｌ，与人类高脂血症接近；油红Ｏ方法染色后在光镜、激光扫描共聚焦显微镜下观察，形态学结果显示实验组小鼠在主动脉窦瓣膜、冠状动脉等部位均形成典型的含有大量泡沫细胞的动脉粥样硬化斑块，Ｒｏｂｅｒｔｓ＆Ｔｈｏｍｐｓｏｎ评分达到８．０±１．２。这表明成功地建立了一种实验性动脉粥样硬化纯系小鼠整体动物模型。

The C57BL/6J mice were assigned at random to two groups; the experimental group was fed cholesterol-rich diets; the control group received normal mouse chow. Mice receiving normal diets were completely free of atherosclerotic lesions. In mice fed cholesterol-rich diets for 16 weeks, the lesions of the aorta and coronary arteries were observed by laser scanning confocal microscopy (LSCM). The typical atherosclerotic plaques, which consisted of amounts of foam cells. were formed near the aortic valves.Roberts & Theompson remarks were 8. 0 ±1. 2. The atherosclerotic lesions were verey similar pathologically to those in the human. The plasma total and free cholesterol levels increased progressively. The obtained results suggested that an atherosclerotic murine model be successfully established. It may provide an avallable experimental model for further investigation into the molecular mechanism of atherosclerosis and screening of antiatherosclerotic drugs.