过敏性休克死亡豚鼠脏器中IgE、IL-4的表达及其法医学意义

Expression and Forensic Significance of IgE and IL-4 in Organs of Guinea Pigs Died of Anaphylactic Shock

【目的】寻找法医学鉴定过敏性休克死亡的病理形态学诊断指标。【方法】利用组织芯片技术采用免疫组化方法(SP法)检测35只过敏性休克豚鼠(实验组28只,对照组7只)死后0、6、12、24h4个时间点的心、肝、肺、肾、脾、胃、肠、气管及扁桃体组织中的IgE及IL-4的表达情况。【结果】IgE的表达:实验组肺及气管组织呈阳性表达,并以0、6h表达最强,各时间点的表达强度有统计学意义,P<0.01;脾组织呈弱阳性表达,各时间点的表达强度无统计学意义,P>0.05;实验组其它脏器及对照组所有脏器均无表达。IL-4的表达:实验组肺、气管及胃组织呈阳性表达,并以0、6h表达最强,各时间点的表达强度有统计学意义,P<0.01;肠组织呈弱阳性表达,各时间点的表达强度无统计学意义,P>0.05;实验组其它脏器及对照组所有脏器均无表达。【结论】免疫组化方法检测IgE、IL-4的表达,可望作为鉴定过敏性休克死亡的病理形态学诊断指标。

[Objective] To seek the pathomorphological targets in forensic identification of anaphylactic shock. [Methods] After 35 guineapigs were died of anaphylactic shock, in which the test group was 28 and the control group was 7, we selected the hearts, lungs, livers, spleens, kidneys, stomachs, intestins, tracheas and tonsils of the guineapigs at 0 h, 6 h, 12 h, and 24 h, respectively and detected the expression of IgE and IL-4 by tissue chip and S-P immunohistochemical method. [Results] （1） The expression of IgE： There was positive expression in the lungs and tracheas in the test group, and was the highest at 0,6 hour in four times, and there were statistical significance, P 〈 0.01; There was low expression in the spleens in the test group, and no statistical difference, P 〉 0.05; but no expression in other tissues in the test group and in all tissues in the control group. （2） The expression of IL-4： There was positive expression in the lungs, tracheas, stomachs, and intestins in the test group, and was the highest at 0, 6 hour in four times, and there were statistical significance, P 〈 0.01;There was low expression in the intestins in the test group, and no statistical difference, P 〉0.05; but no expression in other tissues in the test group and in all tissues in the control group. [Conclusion] To detect the expression of IgE, IL-4 by immunohistochemical method may be hopeful to become the pathomorphological targets for anaphylactic shock.