实验性IgA肾病模型的改进

Improvement of Experimental IgA Nephropathy Model

【目的】对以往的实验性IgA肾病模型的制作方法加以改良,建立一种更加可靠、稳定、成功率高的模型。【方法】清洁型雄性SD大鼠12只,质量220￣260g,按随机数字表随机分为正常对照组、改良造模组。改良组造模方法:口服免疫原牛血清白蛋白(BSA)剂量较常用剂量增加1倍,为400mg/kg,隔天1次灌胃,四氯化碳(CCl4)注射方式由既往的腹腔注射改为皮下注射,用诱导肝纤维化剂量的1/3(皮下注射蓖麻油0.5mL+CCl40.10mL,每周1次,持续9周),并联合运用脂多糖(LPS),第6,8周予以0.05mg尾静脉注射。【结果】改良造模组的6只大鼠均出现蛋白尿,其中4只出现肉眼血尿,2只出现镜下血尿,免疫荧光显示肾组织IgA强度(2+￣3+),光镜高碘酸-希夫染色(PAS)染色显示有弥漫性的轻中度系膜区增生,血白蛋白(ALB)水平较正常对照组明显降低,差异有统计学意义(P<0.05),转氨酶较正常对照组没有升高。【结论】运用改良的造模方法BSA+LPS+CCl4建立IgA肾病模型效果良好,病理、临床指标均与人类IgA肾病较为相似。

[Objective] To improve the method of early constructing IgA nephropathy model and seek more reliable and stable model. [Methods] Twelve clean SD rats （220-260 g） were randomly divided into control group and reforming group. Reforming method： the dose of oral immunogen bovine serum albumin （BSA） increased one times （400 mg/kg every other day）, the method of injecting carbon tetrachloride CCl4 was subcutaneous injection instead of intraperitoneal injection, which dose was one third of content to induce hepatic fibrosis （benne oil 0.5 ml./ weekly and CCl4 0.10 mL/weeldy, continue 9 weeks）. Combined with lipopolysaccharide （0.05 mg/once everyday, the sixth and ninth week once）, [Results] In reforming group, the six rats totally emerged albuminuia, 4 rats had gross hematuria, two had micro-hematuria. Inununofluorescence showed renal IgA density was （2＋-3＋）, the sections stained with periodic acid-Schiff （PAS） examined by light microscopy showed diffuse mild-moderate mesangial proliferation, the level of serum albumin （ALB） obviously decreased compared with normal group, there was significantly different （P〈 0.05）. Aminotransferase did not increase compared with normal group. [Conclusion] To apply with BSA, LPS, and CCl4, IgA nephropathy model proves good effect, which pathology and clinical index resemble human IgA nephropathy.