杜仲叶片愈伤组织诱导的激素优化研究

Phytohormones combination on leaf callus induction of Eucommia ulmoides

以杜仲优树L33的幼叶为材料，在B5培养基上添加不同浓度的生长素与细胞分裂素进行愈伤组织诱导研究，结果表明：无激素的培养基上不能诱导出愈伤组织，单独加入2，4-D、NAA和IBA均可诱导出愈伤组织，以0．5mg·L^-12，4-D、0．5～1．0mg·L^-1NAA、1．0mg·L^-1IBA出愈率最高，达100％，且愈伤组织生长较好；在适宜浓度的生长素的培养基上加入细胞分裂素时，KT的加入对愈伤组织的诱导及生长起抑制作用；1．0mg·L^-1NAA＋0．3～0．5mg·L^-1BA与1．0mg·L^-1IBA＋0．3～0．5mg·L^-1BA的组合可明显促进愈伤组织的诱导和生长，适合进一步继代培养。

The leaves of Eucommia ulmoides Oliv. L33 as explants, the B5 being as the basic medium supplemented with auxin, the combination of auxin and cytokinin, the callus induction and growth of E. ulmoides Oliv. were studied. The results showed that, callus wasn＇t formed on the no phytohormones media. Callus was induced when explants were cultured on B5 medium supplemented with 2,4-D, NAA and IBA only. 0.5 mg · L^-1 2,4-D, 0.5 -1.0 mg · L^-1 NAA, 1.0 mg · L^-1 IBA were induced 100 per cent for callus induction, and was good for the growth; When the cytokinin was added in the proper auxin medium, KT was bad for callus induction and growth; The best combination of callus induction and growth was 1.0mg· L^-1 NAA ＋0. 3 - 0.5mg·L^-1 BA or 1.0mg· L^-1 IBA ＋0. 3 - 0.5 mg · L^-1 BA, whose calluses were growth quickly, suitable for subculture.