廊坊杨3号快繁和转基因的再生系统(英文)

In-vitro regeneration of Populus langfangensis 3 for transformation and micropropagation

对杂交杨树新品种廊坊杨3号（Populus langfangensis3，（P．deltoides（“Shan Hai Guan”）×（（P．simonii×pyramidalys）12×Ulmus pumila）的离体叶片和茎段在附加BA、NAA、IBA和2，4-D的MS培养基上的直接和间接的器官分化、愈伤组织形成和植株再生进行了研究。叶柄和叶片最容易在叶脉处直接诱导生芽。从叶片直接诱导生芽的激素条件为1—2mg·L^-1BA和0．5mg·L^-1 IBA，最高生芽率可达90％。2，4-D促进愈伤组织的形成。由愈伤组织诱导生芽的激素条件为0．3—0．5mg·L^-1BA和0．02mg·L^-1IBA或NAA，生芽率达76％。较好的生根条件为0．1mg·L^-1BA和0．2—0．5mg·L^-1IBA，生根率可达67％。以上再生条件为廊坊杨3号的转基因育种和无性快繁技术提供了可能。

The direct and indirect induction of organ differentiation, callus formation and plantlet regeneration from the leaf and stem explants of the hybrid Populus langfangensis 3 （P. deltoides （ ＂Shan Hai Guan＂） × （（P. simonii × pyramidalys ）12 × Ulmus pumila ） were investigated in-vitro on Murashige and Skoog （MS） medium supplemented with various concentrations of benzylaminopurine （BA）, indole-3-butyric acid （ IBA ）, 1-naphthaleneacetic acid （ NAA ） and 2,4-dichlorophenoxyacetic acid （2,4-D）. The petiole and leaf explant was easier for direct shoot regeneration along the veins at cutting sites. Direct shooting efficiency was related to both BA concentration and BA/IBA ratio. Efficient and rapid shooting from the leaf and stem explants was achieved on MS medium containing 1 - 2 mg · L^-1 BA and 0.5 mg · L^-1 IBA. Maximum shooting frequency reached 90%. 2,4-D promoted the callus induction of P. langfangensis 3. The shooting based on callus was obtained on MS medium supplemented with 0.3 - 0.5 mg · L^-1 BA and 0.02 mg · L^-1 IBA or NAA and the corresponding shooting frequency was 76%. Rooting based on callus succeeded on 0. 1 mg · L^-1 BA and 0.2 -0.5 mg · L^-1 IBA, yielding rooting frequency 67%. These regeneration conditions could further be used for micropropagation and transformation of P. langfangensis 3.