温室内长春花总黄酮含量的动态变化研究

Study on variety trends of flavonoids in Catharanthus roseus in greenhouse

以长春花为试材,应用超声提取和分光光度法测定和分析了不同发育阶段长春花不同部位总黄酮含量的动态变化。结果表明:长春花不同时期各部位总黄酮含量由高到低的排列顺序是:果、叶、花、茎、根;不同叶位总黄酮含量是花叶大于果叶,果叶大于营养叶,不同枝位总黄酮含量是果枝大于叶枝,叶枝大于茎,形成由上到下的递减趋势;侧枝各部位总黄酮含量高于主枝,须根总黄酮含量高于主根,形成由外到内的递减趋势。

By using ultrasonic extraction and spectrophotometry methods, changes of flavonoids content in Catharanthus roseus during different developmental periods were studied. The results showed that, The sequence of the flavonoids content in different parts of C. roseus during every periods from highest to lowest is following： fruit, leaf, flower, stem, root. The sequence of flavonoids content of leaves on three different positions from the highest to the lowest is following： flower leaves, fruit leaves, nutrition leaves, the sequence of flavonoids content of branches on three different positions from the highest to the lowest is following： fruit branch, leaf branch, stem, which form a top-to-down degressivn trend. The total flavonoids content of all side branches was higher than that of main branch. In contrast with tap root, the flavonoids content of fibrous roots were higher. Which form a outer-to-inner descending trend.