摘要
实验以菜豆叶子为材料，定量比较了不同叶际微生物提取方法的提取效果，为叶际微生物的研究提供有效的手段。通过实验研究得出，叶际微生物用不同的提取方法和提取溶液提取效果有很大的差别。用超声波提取，随着超声时间的延长，微生物数量先会有一个增长的过程达到最佳效果后又会减少，最佳提取时间为4min左右；水和磷酸缓冲液两种提取溶液的提取效果没有显著的差别；匀浆的提取方法与超声波提取法相比，提取量有明显增多甚至多出一倍左右，前者最大提取量为1．12×10^7个／g鲜叶；而后者为7．67×10^6个／g鲜叶；叶子的采样量一般是在4—5g为宜。通过实验还得出随着叶龄的增大叶际微生物数量会显著的增多，但对于叶际微生物的研究还要综合考虑其他各种因素，随具体的目的才能确定最适合的叶龄。
The extraction methods of microorganisms from the bean＇ s leaves were investigated and the efficiency of different methods was compared. Results showed that different extraction methods and solvents led to quite different results. By ultrasonic cleaner method, the number of microorganisms increased to a maximum with the time prolonging and then decrease, the most effective treatment time is about four minates. There are no significant difference between sterile water and phosphate buffer. Comdance of microbial population significantly, but the leaf age of a sample for a specific purpose still depends on the other factors.
出处
《植物研究》
CAS
CSCD
北大核心
2006年第2期233-237,共5页
Bulletin of Botanical Research
关键词
叶际
微生物
提取方法
phyllosphere
microorganism
extraction method
