eotaxin-3基因多态性与变应性哮喘的相关性

The research on the correlation between eotaxin-3 gene polymorphisms and allergic asthma

目的研究eotaxin-3基因多态性与变应性哮喘的相关性。方法用聚合酶链反应-单链构象多态性-四引物聚合酶链反应-限制性酶切的方法对湖北地区汉族成人eotaxin-3+77C/T和+2497T/G单核苷酸多态性与哮喘易感性、嗜酸性粒细胞(eosinophil,EOS)计数以及血浆总IgE水平的相关性进行了分析。结果eotaxin-3+2497位哮喘组与对照组G等位基因的频率,哮喘组IgE浓度及EOS数量差异有统计学意义,P值分别为0.011、0.021和0.029;+77位哮喘组与对照组T等位基因的频率,哮喘组IgE浓度差异无统计学意义,P值分别为0.824和0.473;+77位哮喘组EOS数量差异有统计学意义,P值为0.044。结论eotaxin-3+2497T/G多态性与哮喘易感性、EOS数量及IgE水平相关,+77位C/T基因多态性与哮喘EOS数量相关。

Objective To study in the linkage between eotaxin-3 gene polymorphisms and allergic asthma susceptivity, blood plasma IgE or peripheral blood eosinophil in adult population of Han nationality from Hubei province of China. Methods Polymerase chain reaction （PCR）, single strand conformation polymorphism （SSCP）, tetra-primer PCR technique and restriction analysis were applied to identify the single nucleotide polymorphism. Results The allele frequency of eotaxin-3 ＋ 2497 G, total levels of plasma IgE and peripheral blood eosinophil counts revealed the significant difference between control and allergic asthma group, that the P value was 0.011, 0.021 or 0.029 respectively. The allele frequency of eotaxin-3 ＋ 77 T and total levels of plasma IgE showed to have no significant difference between control and allergic asthma group, that the P value was 0.824 and 0.473 respectively. However, the peripheral blood eosinophil counts was significantly different between control and allergic asthma group, and the P value was 0. 044. Conclusion Single nucleotide polymorphism of eotaxin-3 ＋ 2497 is associated with the asthma susceptibility, peripheral eosinophil counts and total levels of plasma IgE in adult population from Hubei province, and polymorphism of ＋ 77 is associated with peripheral eosinophil counts.