纤维蛋白原β基因-148C/T多态性与脑梗死的关系

A linkage between β-fibrinogen gene -148C/T polymorphism and cerebral infarction

目的探讨纤维蛋白原(fibrinogen,Fg)β基因启动子区-148C/T多态性、血浆Fg水平与急性动脉粥样硬化性脑梗死的关系。方法用多聚酶链反应-限制性片段长度多态性方法对151例脑梗死患者和101名健康对照进行Fgβ-148C/T基因多态性分析,并应用凝血酶原时间衍生法检测血浆Fg水平。结果脑梗死组血浆Fg水平明显高于对照组(P<0.01);T等位基因携带者较CC基因型者血浆Fg水平明显增高(P<0.01);按性别分组后差异仍有统计学意义;按年龄段分组后,病例组各组T等位基因携带者较CC基因型者血浆Fg水平增高(P<0.05)。中年梗死组较中年对照组T等位基因频率的差异有统计学意义(P<0.05)。结论Fgβ-148C/T基因多态性影响血浆Fg水平,T等位基因可能独立或通过与其它血栓危险因素或环境因素协同作用增高血浆Fg水平,成为脑动脉血栓形成的危险因素。

Objective To study the linkage between - 148C/T polymorphism of β-fibrinogen gene and plasma flbrinogen levels in patients with acute cerebral infarction. Methods One hundred and fifty-one patients with cerebral infarction and 101 healthy individuals were enrolled in this trial. The β-fthrinogen gene - 148C/T polymorphism was analyzed by PCR-restriction fragment length polymorphism, and plasma fibrinogen levels were obtained from prothrombin time assay. Results Plasma fibrinogen levels of patients were significantly higher than those of controls （ P 〈 0.01 ）. In both groups, T allele carriers had higher plasma fibrinogen levels than other those did （ P 〈 0.01 ） ; and the fibrinogen level difference was still significant if both groups was based on their sex （ P 〈 0.05 ）. Divided by age, each group of the study cases has significant difference between two genotypes （ P 〈 0.05）. T - 148 allele frequency of the middle age case in study group was higher than that in control group （ P 〈 0.05）. Conclusion High plasma fibrinogen level is a risk factor to cerebral infarction. Plasma fthrinogcn level is affected by - 148C/T polymorphism of β-fibrinogen gene. With or without other risk factors and environmental factors affecting, T allele increases plasma fibrinogen level and may be a heritable risk factor to cerebral infarction.