姬松茸多糖的微波辅助提取与含量测定

Microwave- assisted Extraction and Content Determination of Polysacccharide in Agaricus blazei Murill

采用提取时间、微波功率、液料比的单因素试验和三因素三水平正交试验优化了微波辅助提取姬松茸多糖的条件,并用苯酚—硫酸法对姬松茸多糖含量进行了测定。结果表明:以水为溶剂,姬松茸多糖最佳提取条件为:提取时间30min,微波功率640W,液料质量比20∶1。姬松茸多糖换算因子为1.479,多糖含量为13.095%,用该方法测定糖含量的相对标准偏差(RSD)为2.329%。

The conditions of polysaccharides extraction for Agaricus blazei Murill including extraction time, microwave power and water / dry weight ratio were studied by single factor experiment and orthogonal design, and the contents of polysaccharide in Agaricus blazei Murill were measured by phenol-sulphate acid method.The results showed that the optimum extraction conditions were as follows： 20min, 80% microwave power （full power is 800W）, 20：1 （water / dry weight）, where the converting factor of polysaccharide in Agaricus blazei Murill was 1.479 and its polysaccharide content was 13.095%, and the RSD was 2.329%.