NF-κB和Caspase-3在金雀异黄素诱导人胃癌SGC-7901细胞凋亡中的作用研究

Study on Mechanisms of Human Gastric Carcinoma Cells Apoptosi Induced by Genistein

采用MTT法、形态学观察和琼脂糖凝胶电泳法检测了金雀异黄素(genistein,Gen)对体外培养的人胃癌SGC-7901细胞的生长和凋亡诱导作用,并采用免疫组化法和WesternBlot法检测Gen对人胃癌细胞NF-κB、Caspase-3蛋白的表达情况。结果显示:Gen可抑制肿瘤细胞生长,诱导细胞凋亡,抑制NF-κB蛋白表达,增加Caspase-3蛋白表达。结果提示:Gen抑制NF-κB蛋白表达、增加Caspase-3蛋白表达,可能是其诱导胃癌细胞凋亡的机制之一。

The apoptosis in gastric cancer cells induced by genistein, and the relation between this apoptosis and the expression of Caspase-3 and NF-kB were investigated, in vitro experiments, MTT assay was used to determine the cell growth inhibitory rate. Transmission electron microscope and DNh-fragment assay were used to detect the apotosis status of gastric cancer cell lines SGC-7901 before and after the genistein treatment. Immunohistochemical staining and Western Blotting were performed to detect the expression of gene NF-kB and Caspase-3. The results showed that the genistein inhibited the growth of gastric carcinoma cell line SGC-7901 in a dose-and time-dependent manner. Genistein induced SGC-7901 cells to undergo apoptosis with typically apoptotic characteristics, including morphological changes of chromatin condensation, chromatin crescent formation, nucleus fragmentation and apoptotic body formation. Genistein could reduce the protein expression of NF-kB, and improve the protein expression of Caspase-3. The results suggested that genistein was able to induce the apoptosis in gastric cancer. This apoptosis may be mediated by downexpression of gene NF-kB and upexpression of gene Caspase-3. The inhibition of the expression of NF-kB and enhance of the protein expression of Caspase-3 would be apossible mechanism.