延胡索乙素对四氯化碳致小鼠肝损伤的保护作用

Protective effect of dl-tetrahydropalmatine on liver injury induced by carbon tetrachloride in mice

目的:研究延胡索乙素对小鼠肝损伤的保护作用。方法:建立四氯化碳诱导小鼠肝损伤模型,以测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和肝组织丙二醛(MDA)含量和肝组织超氧化物歧化酶(SOD)活性以及肝组织形态变化为指标观察延胡索乙素对肝损伤的保护作用。结果:延胡索乙素能明显降低肝损伤小鼠的血清ALT,AST和肝匀浆MDA含量,肝匀浆SOD活性提高,肝组织变性明显减轻,肝组织结构完好。结论:延胡索乙素具有明显的保护肝脏作用,其机制可能与其抗氧化作用有关。

Objective： To study the protective effect of dl-tetrahydropalmafine（dl-THP） on liver injury induced by carbon tetrachloride （CCl4） in mice. Method： Mice were administracted with dl-tetrahydropalmatine ip 20, 40 mg·kg^- 1 daily for 9 d respectively, and then actue liver injury model was induced by 0.1% carbon tetrachloride ip 20 mL·kg^- 1 The mice were killed 17 h after injection ip of CCl4, serum alanine and aspartate aminotransferase （ALT and AST） activity were measured, and maleic dialdehyde （MDA） and superoxide dismutase（SOD） activity in liver were detected. Result： dl-THP significantly reduced the level of serum ALT and AST, inhibited lipoperxidation in liver, while increased SOD activity in liver tissue. Degeneration of hepatocytes was obviously prevented in mice treated with dl-THP, and the liver histological structure was well maintained. Conclusion： dl-THP has inhibitory effects on liver injury induced by CCl4 in mice. The mechanisms may be related with its effects of reducing lipid peroxidation product.