淫羊藿总黄酮对大鼠成骨细胞代谢调控的机制研究

Effect of Herba Epimedii flavone on the osteoblasts metabolism in vitro

目的:探讨淫羊藿总黄酮对大鼠成骨细胞代谢调控的影响。方法:取新生SD大鼠的颅骨,胶原酶法培养成骨细胞。应用MTT法、对硝基苯磷酸盐法(PNPP)和RT-PCR方法观察淫羊藿总黄酮对体外培养成骨细胞的增殖、碱性磷酸酶活性和OPG mRNA,RANKL mRNA表达的影响。结果:淫羊藿总黄酮能刺激成骨细胞增殖,提高碱性磷酸酶活性,增强OPG mRNA的表达(P<0.05或P<0.01)。结论:淫羊藿总黄酮能促进成骨细胞的增殖、分化,增加OPG mRNA的表达,对RANKL的表达无明显影响。

Objective： To explore the effect of Herba Epimedii flavone（HEF） on the osteoblast metabolism in vitro. Method： Osteoblast were obtained firm new born rat calvaria by digestive enzymes. MTT, PNPP and RT-PCR were used to observe the proliferation, activity of ALP and mRNA expression of OPG and RANKL of cultured osteoblasts in vito. Result： It was found that HEF had the effect on stimulating cell proliferation, activity of ALP and the mRNA expression of OPG of cultured osteoblasts （ P 〈 0.01, P 〈0.05）. Conclusion： HEF can promote the proliferation, the differentiation and the expression of OPG mRNA of the osteoblasts cultured in vitro.