摘要
一株野生曲霉(Aspergillussp.JN19)所产果糖转移酶为胞内酶,该酶的最适反应温度为50℃,在45～52℃范围内能够保持较高的酶活性;反应的最适pH为5.5,pH在4.0～6.0的范围内酶活力稳定;Mg2+对该酶具有激活作用,可以使酶活力提高2.2倍,Cu2+则完全抑制果糖转移酶活力;该酶能够催化蔗糖分子中的果糖基转移形成新的低聚糖;该酶具有广泛的受体特异性。
The result indicated that the fructosyltransferase from a wild strain ofAspergillus sp.JN19 was a intracellular enzyme. The optimum temperature and pH for the high activity of fructosyltransferase were 50℃ and 5.5, respectively. Mg^2＋ ions enhanced fructosyltransferase activity by 2,2-fold whereas Cu^2＋ ions completely inhibited enzyme activity. The enzyme synthesized new fructosyl oligosacchrides by transfering fructose moiety of sucrose to various acceptors.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第3期79-82,共4页
Food Science
关键词
低聚糖
果糖转移酶
底物特异性
oligosaccharides
fructosyltransferse, substrate specifity
