金属离子对红发夫酵母的生长、细胞形态及虾青素合成的影响

Effects of Metal Ions on Cell Growth, Cell Morphology and Astaxanthin Biosynthesis of Phaffia rhodozyma

主要研究了Mn2+、Mg2+和Ca2+三种金属离子对红发夫酵母(Phaffiarhodozyma)的生长、细胞形态及虾青素生物合成的影响。结果表明,在种子培养基中添加Mn2+能明显提高红发夫酵母的β-D-葡萄糖苷酶的活性并改变细胞的形态;在发酵培养基中添加0.1g/LMn2+,能明显促进细胞生长及虾青素合成;用正交试验法优化得到发酵培养基中的金属离子浓度为:0.1g/LMn2+、0.7g/LMg2+、0.2g/LCa2+,在优化后的培养基中,细胞生长速度快,摇瓶发酵57h的虾青素产量达2.95mg/L,是不加金属离子情况下发酵产虾青素最大量(发酵72h)的1.31倍。

The effects of different metal ions on cell growth, cell morphology and astaxanthin biosynthesis of Phaffia rhodozyma were studied. It indicated that Mn^2＋ showed clear positive effects on the β-D-glucosidase activity and cell morphology in seed culture medium. The initial ferment medium containing 0.1g/L Mn^2＋ could improve obviously the cell growth and astaxanthin biosynthesis. The optimal conditions obtained by orthogonal experiment were： 0.1g/L Mn^2＋, 0.7g/L Mg^2＋ and 0.2g/L Ca^2＋ in the ferment medium. Under the optimal conditions, the cell grows rapidly, and the astaxanthin yield reaches 2.95mg/L at 57 h, which is 1.31 times of that under no added metal ions （at 72 h）.