水果、蔬菜中乙烯利残留量的测定方法

Assay Method of Ethephon Residue in Fruits and Vegetables

乙烯利是一种人工合成的植物激素,广泛用于水果和蔬菜的催熟,同时,也易造成食品中的残留污染。为此,研究提出了一种用顶空气相色谱法测定蔬菜、水果中乙烯利残留量的较简便和快速的方法。样品置于顶空瓶中,添加碱液后立即密封加热,吸取样品顶空气体,用气相色谱仪和氢火焰离子化检测器测定,标准曲线法定量。本方法添加回收率在99.1%～105.0%之间;变异系数为8.64%～10.20%;最小检出限为0.01mg/kg。适于水果、蔬菜中乙烯利残留量的检测。

Ethephon was applied widely to accelerate the ripening of fruit and vegetable as a plant-growth regulator. But at the same time, it easily became the residue contamination in food. A simple and fast determination method of ethephon residue in food was therefore developed by means of the headspace gas chromatography. The sample and lye were first loaded in headspace bottle, then the headspace bottle was airproofed and heated up. The headspace gas of the sample was extracted and detected by GC with FID, and the quantitation was made by standard curve. The minimum detection limit was 0.01 mg/kg, the average recovery 99.1% ～105.0%, the relative standard deviation 8.64%～ 10.10%, and the linear range of method 0.01～100mg/kg. This determination method is feasible to test the ethephon residue in fruits and vegetables.