低压离子色谱分离—次黄嘌呤增敏化学发光联用检测亚硫酸盐

Sulfite Determination by Low Pressure Ion Chromatography with Chemiluminescence Sensitized by Hypoxzanthine

本文发现次黄嘌呤对高锰酸钾-鲁米诺-SO3体系化学发光有增敏作用。结合低压离子色谱(LPIC)快速分离测定SO3,并用于实际样品中SO3含量测定。在优化条件下,SO3浓度与化学发光强度在5×10-8～4.8×10-6mol/L范围内成正比(r=0.9977),检出限3.2×10-8mol/L,选定SO3浓度为5×10-7mol/L,进行5次平行测定,RSD为3.25%。2-2-2-2-2-

Sulfite concentration is detected by low pressure ion chromatography （LPIC） with chemiluminescence due to the fact that hypoxanthine can sensitize the depression effect of sulfite on luminol- KMnO4 chemiluminescence reaction. The liner range of sulfite concentration under optimal conditions is 5 × 10^-8～4.8 × 10^-6mnol/L （r=0.9977）with a detection limit of 3.2 × 10^-8mol/L, and RSD as 3.25% by assaying 5 × 10^-7mol/L for five times. This simple, rapid and sensitive method is successfully applied to sulfite determination in food samples.