聚合松香钙固定化淀粉酶的研究

Study on Immobilization of Amylase on Calcium-complexed Polymerized Rosin

研究了聚合松香(PR)及聚乙烯醇接枝聚合松香树脂(PVA-g-PR)的配合物的合成及其对淀粉酶的固定化,考察了固定化酶的性能。实验结果表明,聚合松香钙(PRCa2+)固定化酶具有较好的重复使用性,使用5次后,酶活力为首次固定化酶活力的30.8%;最适作用温度70℃,较游离酶高30℃;最适作用pH值5.0,以淀粉为底物,固定化酶的米氏常数Km为1.69×10-2kg/L,较游离酶Km值2.90×10-2kg/L小。

A series complexes of polymerized rosin （PR） or grafted copolymers of polyvinyl alcohol with PR （PVA-g-PR） were synthesized. These complexed polymerized rosins were used as carriers for immobilizing amylase and their properties were investigated. The experimental results showed that amylase immobilized on calcium-complexed PR （ PRCa^2＋ ） had good reusability, the activity was retained 30.8 % after being used 5 times. Optimum operating temperature was 70 ℃ for immobilized amylase which was 30℃ higher than that of free amylase, Optimum pH value was 5.0 for immobilized amylase and 6.0 for free amylase. K, （ Michaelies-Menten constant） of immobilization amylase was 1.69 × 10^-2 kg/L, which is lower than 2.90 × 10 ^-2 kg/L of free amylase.