柿叶黄酮在双水相体系中的分配行为

Partitional Behaviors of Flavones of Persimmon Leaves in Two-phase Aqueous System

利用双水相体系分离纯化柿叶黄酮。以柿叶黄酮在聚乙二醇(PEG)-盐双水相体系中两相间的分配系数及其上相分配率和得率为目标,探讨了PEG的相对分子质量(MW)及其质量分数、盐质量分数、MgC l2添加量、温度以及pH值对柿叶黄酮在双水相体系中的分配特性的影响。实验表明,分离纯化柿叶黄酮的最佳双水相体系是25%PEG 600与25%(NH4)2SO4,最佳的萃取条件是:pH值11,MgC l2的质量分数为3%,温度25℃。

Effective extraction of flavones from persimmon leaves is an important technique in bioresearch, In the present study, extraction and purification of flavones of persimmon leaves were carried out simultaneously by using an aqueous partitioning method. This two-phase aqueous system was prepared by dissolving suitable amounts of polyethylene glycol （ PEG ） and （NH4 ）2SO4 in aqueous solution. Flavones of persimmon leaves were recovered with optimum high yield and high concentration in the upper polymer-rich phase when 25 % PEG 600 and 25 % （NH4 ）2SO4 were applied. The optimum extraction conditions were pH value 11, MgCl2 3 % and temperature 25℃.