不同温度测定血ECP水平对支气管哮喘的结果评价

Evaluation of ECP Level Determination Under Different Temperature for Asthma.

目的探讨在不同温度条件下,正常健康儿童与支气管哮喘患儿血嗜酸性粒细胞阳离子蛋白(ECP)的含量。方法采用固相双位点酶放大化学发光法,分别对25例正常健康儿童与20例支气管哮喘患儿,在不同温度(25℃和37℃)下收集的血清和血浆标本(EDTA-K2做抗凝剂)进行ECP水平测定。结果不同温度的血清组血清ECP水平分别为,正常对照组10.66±5.21μg/L(x-±s)和17.61±11.60μg/L,哮喘组36.51±21.90μg/L和62.72±32.59μg/L,不同温度之间比较有显著性差异(P<0.001),37℃凝集的血清ECP阳性检出率显著高于25℃;在不同温度的血浆组血浆ECP水平分别为,正常对照组4.69±2.09μg/L(x-±s)和5.96±2.40μg/L,哮喘组为7.40±2.35μg/L和8.05±2.23μg/L,不同温度之间比较没有显著性差异(P>0.05)。在同一温度下,血清标本ECP水平显著高于用EDTA-K2做抗凝剂的血浆标本(P<0.01)。结论温度和抗凝剂对血ECP水平有显著影响,37℃凝集为ECP水平测定的最适温度,日常工作中应制定相应温度下的正常参考值。

Objective To study the influence of the treatments including temperature and anti - coagulation agent （EISFA - K2） during the blood samples collection on the ECP level. Methods The serum and plasma samples were collected from 20 childs with asthma and 25 healthy childs under different temperature （25℃ and 37% ） . ECP levels were determined by using immobile double - site enzyme amplified chemiluminescence immunoassay. Results The serum ECP level of the treatment under 37℃ was significantly high （P 〈 0.01） than that under 25~C. The plasma ECP levels of the treatment under 37℃ and 25℃ were not significantly different （ P 〉 0.05） . The serum ECP level was significantly higher （P 〈 0.01 ） than that in plasma collected under the same temperature. Conclusion Temperature and anti- coagulation （EDTA - K2） affected the ECP level, and 37℃ was the best temperature for determining it. Therefore the reference values for different temperature should be established.