季铵盐型纳米气相二氧化硅衍生物的合成、表征及生物学活性

Synthesis, Characterization and Antimicrobial Activity of Nano-Fumed Silica Derivative with Quaternary Ammonium Salts

以纳米气相Si O2为载体,以γ-氯丙基三甲氧基硅烷作偶联剂,用N,N-二甲基十四烷基叔胺进行了季铵化反应制备季铵盐型纳米气相二氧化硅衍生物。用红外光谱,DSC和激光粒度Zeta电位仪对纳米气相二氧化硅季铵盐衍生物进行了表征和测定,用悬菌定量实验(Suspension quantitative test)研究了其抗菌性能。结果表明(1)γ-氯丙基三甲氧基硅烷通过与纳米气相二氧化硅表面羟基的反应键合到二氧化硅表面,随着γ-氯丙基三甲氧基硅烷的用量增加,纳米气相二氧化硅表面羟基被取代程度也增加;(2)纳米气相二氧化硅的零电位在pH4.8,纳米气相二氧化硅季铵盐衍生物的零电位在pH10.5,季铵盐的引入使其零电位发生了较大的位移;(3)所制备的纳米气相二氧化硅季铵盐衍生物对大肠杆菌、金黄色葡萄球菌以及白色念珠菌在振荡作用15min后,平均抑菌率分别为99.99%,99.99%和94.12%。

Nano-fumed silica reacted with γ-chlopropyltrimethoxysilane as a coupling agent, and then by quaternization with N,N-dimethyl-n-tetradecylamine, finally the nano-fumed silica derivative with quaternary ammonium salts was obtained. The nano-fumed silica derivative was investigated by Fourier transform infrared spectroscopy （FTIR）, differential scanning calorimeter （DSC）, and Zeta sizer nano series. The antimicrobial properties of the nano-fumed silica derivative against selected microorganisms were tested by the quantitative suspension method. The results revealed that （1） γ-chlopropyltrimethoxysilane can be bound to the surface of the nano-fumed silica. With increasing the amount of γ-chlopropyltrimethoxysilane, the amount of surface hydroxyl groups of nano-fumed silica decreases. （2） The Zeta potential showed that the isoelectric point of the nano-fumed silica and the modified nano-fumed silica is pH 4.8 and pH 10. 5, respectively. The shift of the isoelectric point is due to the quaternary ammonium groups. （3） The obtained nano-fumed silica derivative has significant inhibition effect on the growth of microorganisms, and the bactericidal rates in 15 min for E. coil （8099）, S. aureus （ATCC6538） and C. albieans（ATCC10231） were 99.99%, 99.99% and 94.12%, respectively.