人血清白蛋白与3-氨甲基吡啶相互作用的拉曼光谱研究

Raman Spectroscopic Study of Human Serum Albumin Interacting with 3-Picolylamine

测量人血清白蛋白和一种新型仿生配基3-氨甲基吡啶与人血清白蛋白复合物的拉曼光谱。Raman光谱表明3-氨甲基吡啶与人血清白蛋白结合导致人血清白蛋白的构象及其结构的变化。人血清白蛋白的主链构象主要是α-螺旋结构,3-氨甲基吡啶结合不改变其二级结构,但二硫键的构象产生改变,唯一的色氨酸残基外环境由亲水变为疏水,酪氨酸所处的外环境也发生改变。

Raman spectra of human serum albumin （HSA） and HSA-3-picolylamine complex were obtained. The spectra indicate the configuration and structural transformations of HSA. The results show that the secondary structure is main α-helix, and the binding of 3-picolylamine doesn＇t change the secondary structure. However, the binding changes the configuration of disulfide bonds, transforms a single tryptophan residue from exposed tryptophan residues to hydrophobic environment, and alters the microenvironment of tyrosine.