用可见分光光度法测定茶薪菇生长发育中几种与基质利用相关的酶活性

Determination of Several Substrate-Decomposition Enzyme Activities of Agrocybe Cylindracea at Different Stages of Development with Spectrophotometer

用可见分光光度法研究了茶薪菇和雪莲菇不同生长阶段的过氧化物酶、漆酶、蛋白酶、淀粉酶和纤维素酶的活性。结果表明两种菇的淀粉酶和过氧化物酶都在菌丝生长期最高。可见这两种酶在营养生长期起较大作用,对子实体形成后的影响很小。它们的蛋白酶活力变化相似菌丝生长期高于子实体生长后期。但两种菇在漆酶和纤维素酶活性方面就有较大差异,从中可看出这两种菇是有一定的生理生化差异的。

In the present paper the authors studied the activities of amylase, peroxidase, laccase, protease and cellulase at different developmental stages of agroeybe eylindraeea and its mutation with the spectrophotometer. The result showed that the activity of amylase and peroxidase in hyphal stage is the highest for the two basidiomyeetes. It can be concluded that these two enzymes are important in vegetative growth stage, but they may have little effect after the appearance of fruit body. The change of their protease is similar： in the hyphal growth stage it is higher than that of mature mushroom. But the activities of laccase and eellulase are very different between the two basidiomyeetes. It is obvious that they are some different in physiology and biochemistry.