斑茅杂交后代的分子鉴定

Molecuar Identification of Genuine Hybrids from the Cross of Saccharum and E. arundinaceus

采用SSR、5SrDNA、Alu-like分子标记技术对甘蔗(Badila)与斑茅杂交后代F1及其回交后代BC1、BC2、BC3等进行分析,结果表明,SSR是斑茅杂交后代真实性鉴定的有效方法,mSSCIR33是较佳引物之一;5SrDNA可用于鉴别斑茅F1,但因其多态性不高,不能用作回交后代真实性鉴定,用于斑茅血缘的检测时,随着回交代数的提高,可靠性降低;Alu-like不适宜用作杂种后代真实性鉴定,但是检测斑茅血缘的较好方法。

Molecular markers such as SSRs, 5S rDNA and Alu-like were used to identify genuine progeny from Saccharum X E.arundinaceus and the backcross descendants in this study. The results showed that Microsatellite marker analysis is a reliable method and mSSCIR33 is a good prime pair for hybrid identification among the progenies. Although the 5S rDNA PCR is efficient in ＇identifying F, progeny, its low level of polymorphism within these two species means it is not suitable for identifying hybrids from the backcross, it is not a reliable method of verifying whether the pedigree of E.arundinaceum existed among the putative hybrids from the backcross. Alu-like PCR method is fast and efficient in verifying whether the pedigree ofE. arundinaceus existed in putative hybrids, but it is not suitable for identifying hybrids, as Alu-like band is monomorphic.