多发性创伤患者细胞因子变化及临床意义

Changes of cytokine index in patients with multiple trauma and its clinical significance

目的:探讨多发性创伤患者细胞因子的变化及临床意义。方法:采用放射免疫法和ELISA法检测62例多发性创伤患者入院时、入院后第12小时,1、2、3、7、14天(ISS≥10)血中一氧化氮(NO)、肿瘤坏死因子α(TNF-α)及白细胞介素4(IL-4)水平变化,并与正常健康人进行比较。结果:多发性创伤后血中NO、TNF-α和IL-4水平早期即显著高于正常对照组(P<0.05),于伤后第三天后达到高峰,随后逐渐降低,于伤后2周仍显著高于正常。多发性创伤NO、TNF-α及IL-4水平升高幅度与ISS评分呈显著正相关。结论:动态观察多发性创伤患者细胞因子活性的变化有助于病情的评估及预后判断。

Objectlve：To study the changes of eytokines in patients with multiple trauma and its clinical significance.Methods：The concentration of nitric oxide （NO） was detected by colorimetry,the TNF-2 was determined by mdioimmunoassay and IL--4 was measured by enzyme linked immunosorbent assay （ELISA）in 62 patients with multiple trauma at admission and 12 hours,1,2,3,7 and 14 days,and were compared with those in the healthy control group simultaneously.Results：The serum NO.TNF-α and IL--4 in patients with multiple trauma were shown to be significantly higher than those in the control group.The nitric oxide,TNF-α and IL-4 increased at the early stage and reached the peak at 3 days and subsequently decreased gradually in 2 weeks after trauma.The ISS scores of patients were positively correlated with the peaks of nitric oxide,TNF-α and IL-4.Conclusion：Investigating the changes of cytokines in patients with multipie trauma would contribute to evaluate the patient＇s condition and judge the prognosis of this disease.