摘要
Aim To establish a HPLC method for determining five major metabolites of caffeine in the urine,5-acetylamino-6-formylamino-3-methyluracil(AFMU),1-methylxanthine(1X),1-methyluric acid(1U),1,7-dimethyluric acid(17U) and 1,7-dimethylxanthine(17X) and assess the activity of cytochrome P-450 CYP2A6.Methods The contents of five major metabolites of caffeine in the urine were determined by RP-HPLC method.Frequency distribution histogram was drawn by calculating the 17U/(AFMU+1X+1U+17X+17U) and then evaluated the activity of CYP2A6.Results The frequency distribution histograms of CYP2A6 approximately indicated three distinct groups,the cut of point is 0.23 between fast metabolizer and intermediate type.And the cut of point is 0.15 between slow metabolizer and intermediate type.Conclusion The method is simple and rapid,suitable for the determination of metabolites of caffeine in urine.The method can be used to assay the activity of CYP2A6.
Aim To establish a HPLC method for determining five major metabolites of caffeine in the urine, 5-aeetylamino-6-formylamino-3-methyluracil （ AFMU ） , 1 -methylxanthine （ 1X）, 1 -methyluric acid （1U）, 1,7-dimethyluric acid （17U） and 1,7-dimethylxanthine （17X） and assess the activity of cytochrome P-450 CYP2A6. Methods The contents of five major metabolites of caffeine in the urine were determined by RP-HPLC method. Frequency distribution histogram was drawn by calculating the 17U/ （AFMU ＋ 1X ＋ 1U ＋ 17X ＋ 17U） and then evaluated the activity of CYP2A6. Results The frequency distribution histograms of CYP2A6 approximately indicated three distinct groups, the cut of point is O. 23 between fast metabolizer and intermediate type. And the cut of point is O. 15 between slow metabolizer and intermediate type. Conclusion The method is simple and rapid, suitable for the determination of metabolitcs of caffeine in urine. The method can be used to assay the activity of CYP2A6.
出处
《药学学报》
CAS
CSCD
北大核心
2006年第3期282-284,共3页
Acta Pharmaceutica Sinica
基金
山东省自然科学基金资助项目(Q99C05).
