IL-1B基因多态性、幽门螺旋杆菌感染和慢性萎缩性胃炎关系的研究

Study of the Relation of Interleukin-1B Gene Polymorphism and H.Pylori Infection on Chronic Atrophic Gastritis

目的通过研究IL-1B-511基因多态性对幽门螺旋杆菌(Helicobacter pylori,H.pylori)感染后胃黏膜萎缩、慢性萎缩性胃炎的影响,探讨IL-1B基因多态性与胃癌发生的可能机制。方法(1)采用PCR-限制性长度片段多态性(RFLP)分析法检测胃癌低发区广东省普通人群192例的基因型;(2)采用酶联免疫吸附法(ELISA)检测上述人群的Hp感染率、胃蛋白酶原I(PGl)、胃蛋白酶原Ⅱ(PGII)和胃泌素(Gastrin)的浓度。结果Hp阳性者PGI/PGII显著低于Hp阴性者(P<0.01),Hp阳性的IL-1B-511T/T基因型者PGI/PGII比值显著低于C/C和T/T基因型者(P均<0.05)。血清胃泌素浓度与IL-1B-511的基因型没有明确的关系(P>0.05)。结论在胃癌低发区,IL-1B-511基因型可能增加感染H.pylori后胃黏膜萎缩、慢性萎缩性胃炎发生和发展的危险性。

Objective： To investigate interleukin-1B gene polymorphism effect the mucosal atrophy and chronic atrophic gastritis with H. pylori infection. Methods： （1） IL-1B gene polymorphisms of common people were analyzed by PCR-RFLP. （2）The H. priori infection rate, PG Ⅰ, PGⅡ and gastrin were examined using ELISA or RIA. Results： In low prevalent region people with H. priori infection have higher PG I level, but lower PG Ⅰ/PG Ⅱ ratio, compared with individuals without H. priori infection. IL-1B-511 T/T genotype individuals with H, priori infection have lower PG I/PGII ratio than the C/C and T/T genotype H. priori individuals, the gastrin level was similar among each genotype in IL-1B-511 locus no matter whether H. priori infected or not. Conclusion： In low-prevalent region, IL-1B-511 T/Tgenotype was a risk factor for mucosal atronhv and atronhic gastritis after H. pylori infection