体外培养肺泡Ⅱ型细胞Flk-1受体表达的研究

Flk-1 receptor expression in primary culture of type two alveolar epithelial cells

目的:研究体外培养的肺泡Ⅱ型细胞(AECⅡ)VEGF,F lt-1,F lk-1,SP-B的表达。方法:体外培养AECⅡ,采用免疫组织化学方法和电镜鉴定细胞,然后应用SABC法检测AECⅡ的VEGF,F lt-1,F lk-1,SP-B的表达。结果:AECⅡ培养72 h,F lt-1,F lk-1,VEGF阳性表达,SP-B阴性表达。结论:体外培养的AECⅡ,表达F lt-1、F lk-1等受体,人工干预应用VEGF可能发生一系列信号转导,为医学研究应用VEGF提供理论依据。

Objective To investigate the expression of VEGF, Fit - 1, Flk - 1 and SP - B in primary culture of type two alveolar epithelial cell （ AECⅡ ）. Methods AEC Ⅱ was cultured in vitro and characterized with immunohistochemistry and electronic microscopy.The expression of VEGF, Fit - 1, Fik - 1 and SP - B was detected with SABC method. Results The expression of Fh - 1 ,Flk - 1 and VEGF was observed, the SP - B expression was negative. Conclusion Cultured AEC Ⅱ in vitro expresses Fit - 1, Flk -1. Exogenous administration of VEGF to AEC Ⅱ may trigger a series of signal transduction and this study provides a basis clinical application of VEGF.