转基因微藻作为新型生物反应器的研究进展

Recent Progress in a New Bioreactor: Transgenic Microalgae

转基因微藻作为生物反应器生产生物活性物质已成为基因工程研究的热点之一。目前以转基因微藻作为生物反应器的技术并不十分成熟,然而许多科研人员及生物医药公司将微藻作为新型生物反应器生产有用的外源蛋白。本文对微藻生物反应器构建中的有效核转化方法的建立、转基因微藻中所用的启动子、筛选标记、稳定表达等问题进行了综述,同时介绍了目前转化成功的以及未来可能转化成功的有潜在应用价值的微藻。

Transgenic microalgae as a new bioreactor are getting more attractive in producing biologically active proteins. Although biotechnological processes based on transgenic microalgae are still in their infancy, researchers and companies are considering the potential of microalgae as bioreactor to produce value-added metabolites and heterologous proteins for pharmaceutical applications. In this review, the author outlines the method of nuclear transform, promoter, marker and the main difficulties associated with the stable expression in constructing the microalgal bioreactor. In addition, other species have now been successfully transformed, and many others will be transformed in the near future.