摘要
采用碱皂化石油醚提取，Kromasil C18柱分离，以甲醇；氯仿=75：25等度淋洗，二极管阵列检测器检测，测定了强化奶粉中β-胡萝卜素含量．对最佳皂化用碱量、皂化时间、α、β-胡罗卜素及β-胡罗卜素异构体分离条件进行了探索．该方法回收率为91．6％～96．3％，其标准偏差、变异系数分别为1．64％及1．76％．方法简便、快速，精密度及准确度在允许范围内，适用于奶粉中β-胡罗卜素的测定．
A HPLC method for determination of β-carotene in fortified milkpowder was reported. The sample was analyzed on a C18 column with methane： chloroform= 75 ： 25 （V/V） as eluent. The content was 45. 80 μg/100 g. The recovery, standard derivation, coefficient were 93. 14%. 1. 64 and 1. 76% respectively. This analysis method is simple, rapid, accurate and can be used in the quantitative determination of β-carotene.
出处
《分析测试技术与仪器》
CAS
2006年第1期15-19,共5页
Analysis and Testing Technology and Instruments
