棉纤维固定化菊粉酶酶学性质及连续降解菊糖的研究

Properties and continuous hydrolysis of inulin by immobilized inulinase on cotton cloth

无花果曲霉SK004发酵产生的胞外菊粉酶经部分纯化,采用共价键吸附法固定在4-甲苯磺酰氯活化的棉纤维上。以菊糖为底物,固定化酶反应的最适温度为60℃,最适反应pH为5.0。热稳定性及酸碱稳定性几乎没有变化。在装有固定化酶的填充床反应器中,50℃,0.2mL/min流速下,5%菊糖可达到100%水解率,定容产率43g·L-1·h-1;10%菊糖的水解率为70%,定容产率53g·L-1·h-1,操作半衰期19d。

nulinase from Aspergillus ficuum SK004 was immobilized on cotton cloth activated with ptoluenesulfonyl chloride （tosyl chloride）. The optimum temperature and pH for inulin hydrolysis with the immobilized partially purified inulinase were 60℃ and 5.0, respectively. Thermal stability and pH stability had no significant changes compared with free enzymes. Continuous production of fructose from inulin was investigated using the immobilized inulinase in a packed-bed column reactor. At the space velocity of 0.2mL/min,5%（w/v）solution of inuline was completely hydrolyzed and the volumetric productivity was 43g.L^-1-h^-1 at 50℃; the DH of 10% （w/v）solution of inuline was 70% and the volumetric productivity was 53g .L^-1 .h^-1. The operational half-life of the immobilized inulinase was 19 days.