摘要
基于维生素B6对于鲁米诺(Luminol)和高碘酸钾(KIO4)化学发光反应的增敏作用原理,建立了反相高效液相色谱(RP-HPLC)分离柱后化学发光检测VB6的新方法,并成功运用于饮料中VB6的测定。其中在鲁米诺中加入KBr可以大大增敏该反应。该方法测定VB6的线性范围为5.0×10-7～5.0×10-4g/mL,检测限为2.0×10-8g/mL。对1.0×10-5g/mL的VB6进行连续5次测定的相对标准偏差分别为2.8%,回归方程为Y=954.472X+1.488。
A novel high performance liquid chromatograph chromatography （HPLC）-Chemiluminescence （CL） detection method for simultaneous determination of Pyridoxine （VB6） based on the chemiluminescence strength reaction of VB6 with Luminol- KIO4 system was developed. The proposed method has been applied to the determination of V~ in drinks. This HPLC-CL method showed good linearity when it was evaluated simultaneously with VB6 and the working range for the system was 5.0×10^-7-5.0×10^-4g/mL with a limit of 2.0×10^-8g/mL. The relative standard deviations （RSD） （n=5） were 2.8% for V~, with a regression equation of y=954.472×＋ 1.488.
出处
《食品工业科技》
CAS
CSCD
北大核心
2006年第3期181-183,共3页
Science and Technology of Food Industry
基金
重庆市自然科学基金资助(CSTC-2004BB7087)
