摘要
采用琼脂扩散法以Nisin为标准样品,对副干酪乳杆菌(LactobacillusparacaseiHD1.7)产生的肽类抑菌物质效价测定条件进行了研究,结果表明在2号检测培养基中添加0.75%琼脂、以浓度为106cfu/mL枯草芽孢杆菌为指示菌是最佳检测效价的条件,在此条件下测得的肽类抑菌物质的效价为1.6×104IU/g。
The condition of detecting antimicrobial peptides activities that produced by lactobacillus paracasei HD1.7 by agar diffussion assay were studied, the sample was Nisin. The optimal detecting conditions are as follows: No. 2 medium, 0.75% agar concentration, bacillus subtilis suspension concentration of 106cfu/mL. Under this condition, peptides activities is 1.6×10^4IU/g.
出处
《食品科技》
CAS
北大核心
2006年第3期113-115,共3页
Food Science and Technology
基金
黑龙江省教育厅2005年科学技术研究项目(10551250)
关键词
肽类抑菌物质
效价
琼脂扩散法
antimicrobial peptides
activity
agar diffussion assay