摘要
采用琼脂扩散法以Nisin为标准样品,对副干酪乳杆菌(LactobacillusparacaseiHD1.7)产生的肽类抑菌物质效价测定条件进行了研究,结果表明在2号检测培养基中添加0.75%琼脂、以浓度为106cfu/mL枯草芽孢杆菌为指示菌是最佳检测效价的条件,在此条件下测得的肽类抑菌物质的效价为1.6×104IU/g。
The condition of detecting antimicrobial peptides activities that produced by lactobacillus paracasei HD1.7 by agar diffussion assay were studied, the sample was Nisin. The optimal detecting conditions are as follows： No. 2 medium, 0.75% agar concentration, bacillus subtilis suspension concentration of 106cfu/mL. Under this condition, peptides activities is 1.6×10^4IU/g.
出处
《食品科技》
CAS
北大核心
2006年第3期113-115,共3页
Food Science and Technology
基金
黑龙江省教育厅2005年科学技术研究项目(10551250)
关键词
肽类抑菌物质
效价
琼脂扩散法
antimicrobial peptides
activity
agar diffussion assay
