聚合酶链反应扩增38KDa蛋白质基因诊断结核分枝杆菌

POLYMERASE CHAIN REACTION FOR DETECTION OF A 38 KILODALTON PROTEIN GENE OF MYCOBACTERIUM TUBERCULOSIS

应用ＭＴ１和ＭＴ２引物通过聚合酶链反应扩增人型和牛型结核分枝杆菌的３８ＫＤａ蛋白质基因。测定了反应体系的灵敏度（０．１２×１０２ＣＦｕ／ｍｌ）和特异性。对三种不同的ＤＮＡ抽提法作了比较，认为用Ｔｗｅｅｎ２０和ＮＰ４０作为裂解液与ＳＤＳ法比较有显著性差异（Ｐ＜０．０５），且Ｔｗｅｅｎ２０比ＮＰ４０更适合。

polymerase chain reaction for identification of a 38 kilodalton protein gene of mycobacterium tuberculosis was tested by using MT1 and MT2 primers.The sensitivity(0.12× 102 cfu/ml)and specifialiy of the amplification system was detected.The results showed that the extraction of DNA with Tween 20 or NP40 had significant difference as compared with SDS(P<0.05).The extraction of DNA with Tween 20 was better than with NP40.