C-erbB-2、P53和Ki-67在宫颈癌的表达及其临床意义

Expression and Clinical Significance of C-erbB-2、P53 and Ki-67 in Cervical Carcinoma

目的探讨C-erbB-2、P53和Ki-67在宫颈癌组织中的表达及其临床意义。方法采用免疫组化S-P方法检测40例宫颈癌组织中C-erbB-2、P53和Ki-67的表达情况,并选择10例慢性宫颈炎、10例CIN作对照。结果C-erbB-2、P53和Ki-67三者在正常组织中的阳性表达率分别为10.0%、0、10.0%;在CIN组织中的阳性表达率分别为30.0%、10.0%、30.0%;在宫颈癌组织中的阳性表达率分别为67.5%、57.5%、72.5%,从慢性宫颈炎到CIN再到宫颈癌,C-erbB-2、P53和Ki-67阳性表达率显著升高(P<0.05);C-erbB-2、P53及Ki-67在宫颈癌组织中的阳性表达率随着其临床分期及病理分级的升高而升高,但I期与IIa期、G1与G2、G2与G3之间比较差异无显著性(P>0.05),而G1与G3比较有显著性差异(P<0.05);肿瘤直径>4cm者高于直径≤4cm、有淋巴转移者高于无淋巴转移者(P<0.05)。结论C-erbB-2、P53和Ki-67的异常表达在宫颈癌的发生和发展中可能发挥着一定作用,三者的联合检测可作为宫颈癌诊断及预后评估的参考指标。

Objective To explore the expression and clinical significance of C--erbB--2, P53 and Ki--67 in cervical carcinoma. Methods Immunohistochemistry SP method was used to detect the expression of C--erbB--2 ,P53 and Ki--67 protein in 40cases of cervical carcinoma, 10 cases of CIN and 10 cases cervical epithelium. Results The positive rates of C--erbB--2,P53 and Ki--67 in cervical epithelium, CIN and cervical carcinoma were 10. 0%, 30.0 % and 67.5%, respectively, for C-- erbB-- 2 ; 0,10. 0% and 56.0 %, respectively, for P53, and 10.0 %, 20.0 % and 72.5 %, respectively, for Ki- 67. The positive rates of C--erbB--2,P53 and Ki--67 increased significantly from cervical epithelium to CIN,and from CIN to cervical carcinoma （P 〈 0, 05）. There was no relationship between the expression of C--erbB-- 2,P53 and Ki--67with the clinical stage of cervical carcinoma （P 〉 0.05）. The positive rates of C-- erbB--2,P53 and Ki--67 in cervical carcinoma had statistical association with the pathological grade, tumor length and lymph node metastasis （P 〈0.05）. Conclusions The abnormal expression of C--erbB --2,P53 and Ki--67 may be playing an important role in the occurrence and development of cervical carcinoma. The detect of C--erbB--2 ,P53 and Ki--67maybe an index to the diagnosis and prognosis of cervical carcinoma.