氯硝基安定抗惊厥作用耐受及停药前后对大鼠下丘脑室旁核CRHmRNA表达的影响

CRH mRNA expression increased in amygdala-kindled rats with tolerance to and dependence on the anticonvulsant effect of clonazepan

氯硝基安定抗惊厥作用耐受性及依赖性机制目前尚不清楚。近来发现促肾上腺皮质激素释放激素（ＣＲＨ）具有促惊厥作用，并参与应激过程。我们用原位杂交方法观察了氯硝基安定抗大鼠点燃惊厥耐受及停药前后下丘脑室旁核ＣＲＨｍＲＮＡ表达的变化，发现耐受时ＣＲＨｍＲＮＡ表达增强，停药７ｄ时不再变化。提示ＣＲＨ参与了耐受性与依赖性过程。

Mechanisms underlying tolerance to and dependence on anticonvulsant effect of clonazepam are not clear.With in situ hybridization we found CRH mRNA expression increased significantly at paraventricular hypothalamic nucleus in amygdala-kindled rats which had developed tolerance to the anticonvulsant effect of clonazepam,and on day 7 of clonazepam discon tinuation CRH mRNA expression no longer changed.Results indicated that CRH was involved in the development of tolerance to and dependence on the anticonvulsant effect of clonazepam.