洋地黄毒甙体外抗人癌细胞株的作用

Antitumor Effect of Digitoxin on Human Tumor Cell Lines in Vitro

目的：探讨洋地黄毒甙体外抗肿瘤作用。方法：以台盼蓝排染法、中性红活染法和克隆形成实验测定洋地黄毒甙（ＤＴ）对多种人癌细胞株的细胞毒作用。结果：０．０１～１．２５μｇ／ｍｌＤＴ在体外能显著杀伤ＨＬ－６０．Ｋ５６２．ＳＭＭＣ－７７２１，ＳＧＣ－７９０１细胞，并呈剂量依赖性，药物作用２４ｈ．ＩＣ５０分别是０．１３２．０．１８２．０．２６５．０．４８９μｇ／ｍｌ。对正常成纤维细胞株ＨＬＦ的ＩＣ（５０）为４．２８８μｇ／ｍｌ．明显高于癌细胞，对癌细胞具有选择毒性作用。克隆形成实验中，０．０１～１．２５μｇ／ｍｌＤＴ处理１２ｈ，对ＨＬ－６０和ＳＧＣ－７９０１细胞集落形成有明显的抑制作用，ＩＣ５０分别是０．０５６．０．ｌ１９μｇ／ｍｌ。结论：应用洋地黄毒甙及其他Ｎａ＋－Ｋ＋－ＡＴＰ酶抑制剂防治肿瘤是一个值得探讨新方向。

Objective:To investigate antitumor effect of digitoxin in vitro,Objective:The cytotoxic effects of digitoxin on various human tumor cell lines were determined by trypan blue dye exclusion test,neutral-red vital staining method and colony-forming assay.Results:0.01～1.25μg/ml concentrations of digitoxin were able to kill HL-60,K562,SMMC-7721,SGC-790l cells remarkedly in vitro,showing dose dependence.IC50 of these cells exposed to the drug for 24 hours was 0.132,0.182,0.265,0.489μg/ml.respectively,IC50 of digitoxin on the normal fibroblast cell line CHLF was 4.288μg/ml,which is significantly higher than that of cancer cells,indicating its selective toxicity to cancer cells.With colony forming assay,after treated with digitoxin at 0.01～1.25μg/ml for l2 hotirs,the colony formation of HL-60 and SGC-790l cells was inhibited obviously.IC50 was 0.056,0.ll9μg/ml,respectively.Conclusion:Administration of digitoxin and other Na+-K+-ATPase inhibitors to prevent or treat tumors is a new approach worthy of exploring.