逆转录—多聚酶链反应检测柯萨奇B组病毒

Reverse transcription polymerase chain reaction for detection of coxsackievirus B in 298 clinically samples

研究目的用分子生物学方法对柯萨奇Ｂ组病毒（ＣＢＶ）进行检测并探讨与临床症病的关系。研究方法对我院２９８例疑似ＣＢＶ感染的患儿，采用特异性引物进行逆转录－多聚酶链反应（ＲＴ－ＰＣＲ）检测ＣＢＶ。结果检出ＣＢＶ阳性１２７人，阳性率４２．６％。感染与年龄有关，夏秋季多见。８０例健康儿童中，阳性５人，占６．２５％。２０例ＲＴ－ＰＣＲ检测阳性标本用ＥＬＩＳＡ法检测，１７例阳性。结论ＲＴ－ＰＣＲ可快速、敏感、准确地检出标本中的ＣＢＶ。此病毒可侵犯多种器官，引起各种临床症病，有较强的传染性。

Objectives Molecular biology measurement was used to detect the existence of coxsackievirus B(CBV)in specimen and in estigate its relationship with clincal diseases.Methods The 298 patients of being regarded as CBV infection were detected bt reverse transciption polymerase chain reaction (RT-PCR) with specific oligonucleoticle primers,Results The results showed that 127 cases (42.6%) were positive cud the infection was relevant toage,and more visible in summer and autumn. On the other hand, only 5 of 80 healthy children in control group were positive (6.25%). The 17 of 20 positive samples (with RT-OCR ) detected by ELISA were positive.Conclusions RT-PCR assay is a rapid, sensitive and specific method for the detection of CBV. Theresults shawed that CBV can infest many kinds of organs and result in various clinical diseases. It has strong infectivity.