褶合光谱法同时测定克感敏片中三主药含量

Simultaneous determination of 3-components in keganmin by convolution spectrometry

考察褶合光谱法用于不经分离同时测定多成分混合体系各组分含量的可靠性及可行性。方法：以含四种主药及多种辅料的克感敏片为例，扫描获得对照液、校正液和模拟液的吸收图谱后，褶合光谱仪选出方法最佳回收率，在此条件下自动测定样品中各组分的含量。结果：克感敏片中氨基比林、咖啡因、非那西丁的平均回收率及相对标准偏差分别为：（９９．７６±０．１８）％、（１００．０４±０．９５）％、（１００．１６±０．２５）％，由于另一组分扑尔敏的含量太低，无法准确测定其紫外吸收，褶合光谱法亦无法直接给出其含量。结论：褶合光谱法能很好地解决多组分体系不经分离同时定量的问题，且可省去化学分离方法的繁琐操作，但前提条件是白色体系且能准确测定各成分的紫外吸收。

Objective:To investigate the credibility and feasibility of convolution spectrometry in simultaneously determining each of components without separation.Methods:Keganmin,which comprises four ingredients and many addictives,served as an example.From absorption curves of control,correcting and simulating solutions,convolution spectrometer picked out the optimum recovery,and thereafter determined the concentration of all the components.Results:In keganmin pill,the average recovery and relative standard deviation of aminopyrine,caffeine,phenacetin were(99.76±0.18)%,(100.04±0.95)%,(100.16±0.25)%,respectively.UV absorption of another component,i.e.,chlorpheniramine maleate,could not be determined accurately due to its low concentration,and convolution spectrometer could not directly give its results either.Conclusion:Convolution spectrometry can determine every ingredient in a multi-component system and good results are acquired.Tedious chemical procedures of separation can be avoided,but the pre-condition of the method must be that the system is white and precise absorption of every ingredient is available.