大鼠烫伤早期肝线粒体ATP合成酶和腺苷酸激酶活性的改变

The activity changes of ATP synthase and adenylate kinase in rat-liver mitochondria during the early phase after burn injury

为进一步了解大鼠烫伤早期肝线粒体氧化磷酸化偶联增强的机理，应用荧光素和荧光素酶法，以琥珀酸为底物，测定了烫伤３０ｍｉｎ大鼠肝线粒体合成酶和腺苷酸激酶的活性，发现该两种酶的活性与假处理组相比均增高。提示这两种酶活性增高可能与烫伤早期ＡＴＰ形成率增高和氧化磷酸化偶联增强有关。

To understand the mechanism of the increasing oxidative phosphorylation of rat-liver mitochondria during the early phase of burn injury, using luciferin-luciferase method,succinate as a substrate,the activities of ATP synthase and adenylate kinase in liver mitochondria of male Sprague- Dawley rats 30 min after 20 percent full skin thickness burns increased significantly in comparison with those of the sham-burn rats,suggesting that these two enzymes might contribute to the increased rates of ATP formation and oxidative phosphorylation coupling during the early phase after burn injury.