摘要
为建立可靠的大鼠胚胎移植受胚模型，使适用于着床前染毒致畸的机制研究，本文比较输精管结扎绝育和氯丙二醇灌胃绝育雄鼠制备假孕受胚鼠的优劣。结果表明以氯丙二醇绝育雄鼠制备假孕受胚鼠，可简化实验步骤，并明显提高移植胚胎着床率。该法用于着床前胚胎阿司匹林染毒致畸机制研究，在受胚鼠妊娠末期可见胚胎吸收率和畸形率呈剂量依赖性增高，与着床前胚胎的胚泡化率呈负相关，提示该法可成功地用于药源性着床前胚胎异常，排除母体对胎仔发育的影响因素。
To set up a reliable pseudopregnant model of rat embryo transfer for reproductive toxicity study,two different pseudopregnant models were compared in which female rats had mated with vasectomized males and with 3-chloro-1, 2-propanediol sterilized males. The results showed that the pseudopregnant model which had mated with 3-chloro-1, 2-propanediol-treated males as em- bryo transfer recipients could significantly enhance the implantation rate and markedly simplify the experimental steps. The effect of in vivo preimplantation exposure of rat embryo to aspirin (0. 25,0. 50 and 1. 00 g/kg) on development subsequent to embryo transfer into pseudopregnant recipients was investigated. A dose-related increase in resorption and malformation rate was observed as well as a negative correlationship with blastulation of preimplantation embryo on the 4th of gestation. The method seems more suitable for reproductive toxicity mechanism study.
基金
国家自然科学基金资助项目
关键词
生物学模型
阿司匹林
毒性
药物致畸
胚胎移植
Models biological/methods
3-chloro-1
2-propanediol
Aspirin/tox
Embryo transfer
Drug-induced abnomalities
Rat
