苦参碱对人恶性黑素瘤细胞株侵袭能力及乙酰肝素酶mRNA表达的抑制作用

Inhibition of Invasiveness and Expression of Heparanase-mRNA in Human Malignant Melanoma Cell Line A375 by Matrine

目的:探讨苦参碱对人恶性黑素瘤细胞株A375的侵袭能力及其乙酰肝素酶mRNA表达的影响作用。方法:A375细胞体外培养,以0.125 mg/m、l0.25 mg/m l和0.5 mg/m l不同浓度苦参碱进行预处理48 h后,采用半定量RT-PCR检测各组培养细胞HPSE mRNA表达的变化,细胞黏附实验检测细胞黏附能力的改变,M atrigel侵袭实验检测细胞侵袭能力的变化。结果:和对照组比较,各浓度苦参碱处理组A375培养细胞HPSE mRNA的表达和细胞黏附侵袭能力均受到明显的抑制(P<0.01),其抑制效应与药物的浓度呈正相关(组间比较P<0.01)。结论:苦参碱可能通过下调A375细胞HPSE mRNA的表达而显著抑制细胞的黏附侵袭能力,其抑制作用呈剂量依赖性。

Objective ： To investigate the effect of matrine on the invasiveness and expression of heparanase-mRNA in human ma- lignant melanoma cell line A375. Methods ： The A375 cells were treated by matrine in different concentration. The total RNAs were extracted from the cells 48 hours after treatment and then semi-quantitative RT-PCR were performed to evaluate the heparanase-mRNA expression levels. Effect of matrine on adhesion of treated A375 cells was tested by cell-Matrigel adhesion assay. The invasiveness of treated A375 ceils was measured by Matrigel invasion assay. Results： The hepanase-mRNA expression, adhesion and invasiveness of A375 cells treated with matrine of different final concentrations significantly decreased compared with that of the controls（p 〈 0. 01 ）. Besides, the inhibitory effects were signifcantly different when the cells t,,eated with matrine of different concentrations （P 〈 0. 01 ）. Conclusion： By down-regulating the expression of heparanase-mRNA, matrine has a significant inhibitory effect on the adhesion and invasiveness of human malignant melanoma cell line in a dose-dependent manner.