胃癌和癌前病变免疫组化与组化的研究

STUDY OF IMMUNOHISTOCHEMISTRY AND HISTOCHEMITRY IN GASTRIC CARCINOMA AND PRECANCEROUS LESIONS

应用胃癌单克隆抗体ＭＧ７、高铁二胺－阿尔新蓝（ＨＩＤ／ＡＢ）及核仁组成区（ＡｇＮＯＲｓ）等免疫组织化学（免疫组化）与组织化学（组化）染色方法，对９０例胃粘膜及胃癌组织进行研究。结果表明，ＭＧ７在胃癌原发灶及淋巴结转移灶癌细胞的阳性率分别为７６．７％和１００％，萎缩性胃炎为２５％，胃癌与非癌组间有显著性差异（Ｐ＜０．０１）。在各组的病变组织中，ＡｇＮＯＲｓ计数均值随异型程度的加重而递增，胃癌与非肿瘤病变组间有明显的差异（Ｐ＜０．０５）。１６例ＨＩＤ阳性的大肠型肠上皮化生（肠化）病变中有８例ＭＧ７抗原表达，占５０％，在１５例ＨＩＤ阴性的小肠型肠化中，无１例呈ＭＧ７阳性反应，两者之间也有明显差异（Ｐ＜０．０５）。

Ninety cases of gastric mucosa were studied by means of immunohistochemistry with monoclonal antibody MG7 and histochemistry with high iron diamine-alcian blue(HID/AB) and for nucleolar organizer region(AgNORs). These results indicated that the positive rates of MG7 were 76.7%and 100.0%in primary carcinomas of the stomach and in metastatic lesions respectively and 25% in atrophic gastritis. AgNORs count incresed with the increse of atypism in various types of gastric mucosal lesions. A significant difference was found in the average AgNORs count between gastric carcinomas and non-neoplastic lesions. There were expressions of MG7 in 8 out 16 cases of 'colonic type' intestinal metaplasia of gastric mucosa and were in 15 cases of the 'smal intestinal type'. Significant difference was detected as well in AgNORs count between the two groups.