摘要
用免疫抑制剂氢化可的松体外处理胎鼠和新生鼠表皮，观察它对朗格汉斯细胞数量和结构的影响。结果如下：（１）经氢化可的松处理的表皮内郎格汉斯细胞数量减少，细胞结构发生行性变化。（２）图像分析显示，随氢化可的松浓度增大，郎格汉斯细胞数量和ＡＴＰ酶组化染色反应的灰度逐渐下降。
The authors used an immunosuppressive agent hydrocortisone to treat fetal and newborn miceepidermis in vitro and observed its effects on Langerhans, cells. The results were as follows: (1)The number of ATPase positive Langerhans cells decreased significantly in the hydrocortisone treated epidermis, and some deterorative changes occurred in morphology. (2)The image analysis showed that the density of Langerhans cells and the average gray of ATPase-reacting.substance in these cells decreased progressively with the increase of hydrocortisone concentration. (3) After treatment with hydrocortisone ( 1 mg/ml) partial mitochondria and rough endoplasmic reticulum of Langerhans cells exhibited distention. and the deposits of ATPase-reacting substance in cell membrane decreased. The study provided useful morphological data for prolonging allografts survival time in clinic practice.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1996年第3期247-251,共5页
Chinese Journal of Anatomy
关键词
胎鼠
朗格汉斯细胞
图像分析
氢化可的松
fetal mice
newborn mice
Langerhans cell
image analysis
hydrocortisone
