应用锝标记卵巢癌单克隆抗体进行放射免疫显象的研究

TheStudyofRadioimmunoimagingontheNudeMiceBearingHumanOvarianCarcinomabyLa┐belingMonoclonalAntibodywith99mTc

目的：探讨卵巢癌单克隆抗体（单抗）进行放射免疫显象（放免显象），对卵巢癌追踪和治疗的临床价值。方法：应用锝标记卵巢癌单抗，对荷瘤裸鼠进行放免显象（实验组），以应用锝标记正常小鼠免疫球蛋白进行对照（对照组）。并且与经腹腔与经静脉注射单抗进行比较。结果：实验组裸鼠肿瘤部位全部显象，对照组全部不显象，两组肿瘤及非肿瘤组织放射强度与显象结果一致。经静脉注射单抗的放射本底优于经腹腔注射。结论：锝标记卵巢癌单抗进行放免显象特异性强，可用于临床。

Objective:Toinvestigatethefeasibilityofradioimmunoimaging(RI)onthenudemicemodelbylabelinganti-ovariancarcinomamonoclonalantibody(McAb,COC166-9)with99mTc,andthepossibilityforclinicalapplication.Methods:McAbreducedbybeta-mercaptoethanolwaslabeleddirectlywith99mTc.Imagingpositiverate,negativerateandtumor/non-tumor(T/N)ratiowerecalculated.Results:(1)McAbCOC166-9labeledwith99mTcreactedwithovariancarcinoma.(2)8nudemicemodelswereinjectedwiththelabeledantibodiesandwerethenimaged.Intheex-perimentalgroupthepositiveimagingrateoftumorwas100%,inthecontrolgroupthenegativeratewas100%.TheresultswerethesameasthoseofT/Nratios.(3)Intwogroups(10nudemice),in-traperitoneal(IP)orintravenous(IV)injectionofMcAblabeledwith99mTc,positiveimagingratesoftumor100%.TheIVgroupwasbeterthanIPgroupintheradio-background.Conclusions:Im-munoactivityof99mTc-COC166-9usedforRIonthenudemicemodelswasgood,andtheimagingefectwasspecific.Clinicalapplicationispromising.