血虚证动物模型的标准化研究初探

Study on Developing the Standard Animal Model of Blood Deficiency Syndrome

目的建立标准化的血虚证动物模型。方法采用慢性失血法、溶血法和骨髓抑制法制造三种血虚证模型,观察一般情况和与血发生有关的实验室指标,并用四物汤反证造摸效果。结果三种动物模型均符合血虚证表现,同时出现外周血、骨髓有核细胞数目和细胞周期的改变,四物汤对三种动物模型均有一定疗效。结论建立了三种符合标准化的血虚证模型。

Objective To develop the standard animal models of blood deficiency syndrome. Methods Three kinds of blood deficiency syndrome models were developed by blood loss, hemolysis, and arrest of bone marrow, and the symptoms, signs, and laboratory indexes related to hematogenesis were observed. The therapeutic effects of Siwutang （四物汤） were observed as counterevidence in three kinds of model. Results The manifestations of the animals in the three kinds of model were in keeping with clinical manifestations of blood deficiency syndrome, with the changes of peripheral blood, marrow cells and their cell - division period, and Siwutang （四物汤） could exert satisfactory therapeutic effects on the changes. Conclusion Three kinds of standard animal model of blood deficiency syndrome were established in mice.